Crystal structure of a dimeric octaheme cytochrome c3 (Mr 26000) from Desulfovibrio desulfuricans Norway
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Voltammetry of Cytochrome c3 from Desulfovibrio desulfuricans (strain Norway) at the Graphite Electrode
Interfacial reactions of cytochrome c3 at a graphite electrode were studied in a neutral medium by means of the differential pulse and cyclic voltammetry. It was found that ferricytochrome c3 yielded three cathodic peaks G, d i and Cm at potentials of —0.3 to —0.5 V (vs. Ag/AgCl reference electrode) on the voltammograms. Upon anodic polarization this protein yielded two anodic peaks Ai and An a...
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Cytochrome c3 (M(r) 26000) isolated from Desulfovibrio gigas is a dimeric cytochrome consisting of two identical subunits of 109 amino acids, each of which contains four haem groups. On the basis of its amino acid sequence, this cytochrome clearly belongs to the cytochrome c3 superfamily, and will be classified in class III of the c-type cytochromes as defined by Ambler [(1980) in From Cyclotro...
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To explore the physiological role of tetraheme cytochrome c(3) in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The physical analysis of the DNA from the strain carrying the integrated plasmid showed that the insertion was successful. The growth rate of the mutant on lactate with sulfa...
متن کاملFunctional expression of Desulfovibrio vulgaris Hildenborough cytochrome c3 in Desulfovibrio desulfuricans G200 after conjugational gene transfer from Escherichia coli.
Plasmid pJRDC800-1, containing the cyc gene encoding cytochrome c3 from Desulfovibrio vulgaris subsp. vulgaris Hildenborough, was transferred by conjugation from Escherichia coli DH5 alpha to Desulfovibrio desulfuricans G200. The G200 strain produced an acidic cytochrome c3 (pI = 5.8), which could be readily separated from the Hildenborough cytochrome c3 (pI = 10.5). The latter was indistinguis...
متن کاملA single mutation in the heme 4 environment of Desulfovibrio desulfuricans Norway cytochrome c3 (Mr 26,000) greatly affects the molecule reactivity.
The gene encoding Desulfovibrio desulfuricans Norway cytochrome c3 (Mr 26,000), a dimeric octaheme cytochrome belonging to the polyheme cytochrome c3 superfamily, has been cloned and successfully expressed in another sulfate reducing bacteria, D. desulfuricans G201. The gene, named cycD, is monocistronic and encodes a cytochrome precursor of 135 amino acids with an extension at the NH2 terminus...
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ژورنال
عنوان ژورنال: Structure
سال: 1996
ISSN: 0969-2126
DOI: 10.1016/s0969-2126(96)00045-7